Japan on Monday ordered the slaughter of some 42,000 chickens after officials confirmed the country’s second bird flu outbreak in less than a month.

DNA tests confirmed the H5 strain of the virus at a farm in Miyazaki prefecture in the country’s southwest after its owner reported Sunday that several chickens had suddenly died, the local government said.

Officials began the slaughter Monday and asked farms within a 10 kilometre (62 miles) radius not to move their poultry outside of that geographic area.

The case comes about two weeks after the government ordered the slaughter of some 4,000 chickens at another poultry farm in Miyazaki — the first confirmed outbreak of bird flu at a Japanese poultry farm since April.

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